Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP) Degradation by Alkaline Phosphatase*
نویسندگان
چکیده
منابع مشابه
Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a ubiquitous second messenger providing a Ca(2+) trigger in a wide range of cell types. However, its metabolism is not well understood. Here, we demonstrate the presence of endogenous NAADP in HeLa cells. CD38, a promiscuous enzyme described to be involved in NAADP metabolism, was not detectable in HeLa cells. In cell-free extracts of HeL...
متن کاملNicotinic Acid Adenine Dinucleotide Phosphate (NAADP
Microinjection of human Jurkat T-lymphocytes with nicotinic acid adenine dinucleotide phosphate (NAADP 1 ) dose-dependently stimulated intracellular Ca 2 1 -signaling. At a concentration of 10 nM NAADP 1 evoked repetitive and long-lasting Ca 2 1 oscillations of low amplitude, whereas at 50 and 100 nM, a rapid and high initial Ca 2 1 -peak followed by trains of smaller Ca 2 1 -oscillations was o...
متن کاملNicotinic acid adenine dinucleotide phosphate (NAADP+).
Microinjection of human Jurkat T-lymphocytes with nicotinic acid adenine dinucleotide phosphate (NAADP 1 ) dose-dependently stimulated intracellular Ca 2 1 -signaling. At a concentration of 10 nM NAADP 1 evoked repetitive and long-lasting Ca 2 1 oscillations of low amplitude, whereas at 50 and 100 nM, a rapid and high initial Ca 2 1 -peak followed by trains of smaller Ca 2 1 -oscillations was o...
متن کاملNicotinic Acid Adenine Dinucleotide Phosphate (NAADP)-mediated Calcium Signaling*
Nicotinic acid adenine dinucleotide phosphate (NAADP)2 is a metabolite of NADP that was first identified as the most potent Ca2 stores mobilizing molecule in sea urchin egg homogenates more than a decade ago (1, 2). It has since been shown to be effective in a wide variety of cells, from plant to animal, including human (reviewed in Refs. 3–5). Its mechanism of action is distinct from those of ...
متن کاملDetermination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca2+-mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior incubation with low concentrations of NAADP is more effective in inhibiting subsequent binding of radiolabelled NAADP than incubating the preparation with the two ligands s...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2012
ISSN: 0021-9258
DOI: 10.1074/jbc.m112.362715